stencil-micropatterning-human-pluripotent-stem-cells-for-probing

Stencil Micropatterning of Human Pluripotent Stem Cells for Probing Spatial Organization of Differentiation Fates

Human pluripotent stem cells (hPSCs), including embryonic stem cells and induced pluripotent stem cells, have the intrinsic ability to differentiate into ...

a-high-throughput-platform-for-culture-and-3d-imaging-of-organoids

A High-Throughput Platform for Culture and 3D Imaging of Organoids

The characterization of a large number of three-dimensional (3D) organotypic cultures (organoids) at different resolution scales is currently limited by ...

National University of Singapore

A novel thermal decomposition approach to synthesize hydroxyapatite-silver (Hap-Ag) nanocomposites has been reported. The nanocomposites were characterized by X-ray diffraction, field emission scanning electron microscopy coupled with energy dispersive X-ray analysis, transmission electron microscopy and diffuse reflectance spectroscopy techniques. Antibacterial activity studies for the nanocomposites were explored using a new rapid access method employing recombinant green fluorescent protein (GFP) expressing antibiotic resistant Escherichia coli (E. coli). The antibacterial activity was studied by visual turbidity analysis, optical density analysis, fluorescence spectroscopy and microscopy. The mechanism of bactericidal action of the nanocomposites on E. coli was investigated using atomic force microscopy, and TEM analysis. Excellent bactericidal activity at low concentration of the nanocomposites was observed which may allow their use in the production of microbial contamination free prosthetics.

A novel thermal decomposition approach to synthesize hydroxyapatite-silver nanocomposites and their antibacterial action against GFP-expressing antibiotic resistant E. coli.

Human pluripotent stem cells (hPSCs) have the intrinsic ability to differentiate and self-organize into distinct tissue patterns, although this requires the presentation of spatial environmental cues, i.e., biochemical and mechanical gradients. Cell micropatterning technologies potentially offer the means to spatially control stem cell microenvironments and organize the resultant differentiation fates. Here, we describe stencil micropatterning as a simple and robust method to generate hPSC micropatterns for controlling hPSC differentiation patterns. hPSC micropatterns are specified by the geometries of the cell stencil through-holes, which physically confine the locations where the underlying extracellular matrix and hPSCs can access and attach to the substrate. This confers the unique capability of stencil micropatterning to work with a variety of culture substrates and extracellular matrices for optimal hPSC culture. We present the detailed steps of stencil micropatterning to successfully generate hPSC micropatterns, which can be used to investigate how spatial polarization of cell adhesion results in cell fate heterogeneity.

Geetika Sahni

Department of Biomedical Engineering,
Mechanobiology Institute (MBI)

National University of Singapore

Stencil Micropatterning of Human Pluripotent Stem Cells for Probing Spatial Organization of Differentiation Fates

A High-Throughput Platform for Culture and 3D Imaging of Organoids

Geetika Sahni

.css-o250i3{font-size:18px;font-family:Open Sans,sans-serif;line-height:21.6px;}Department of Biomedical Engineering,Mechanobiology Institute (MBI)

National University of Singapore

Stencil Micropatterning of Human Pluripotent Stem Cells for Probing Spatial Organization of Differentiation Fates

A High-Throughput Platform for Culture and 3D Imaging of Organoids

Department of Biomedical Engineering,
Mechanobiology Institute (MBI)