Philip E. Johnson

Assembly PCR oligo maker: a tool for designing oligodeoxynucleotides for constructing long DNA molecules for RNA production.

RNA recognition by the Vts1p SAM domain.

The three-dimensional structure of the Moorella thermoacetica selenocysteine insertion sequence RNA hairpin and its interaction with the elongation factor SelB.

Enhanced NMR signal detection of imino protons in RNA molecules containing 3' dangling nucleotides.

Structure of the cytosine-cytosine mismatch in the thymidylate synthase mRNA binding site and analysis of its interaction with the aminoglycoside paromomycin.

Defining a stem length-dependent binding mechanism for the cocaine-binding aptamer. A combined NMR and calorimetry study.

Identification of RNA-ligand interactions by affinity electrophoresis.

Defining the secondary structural requirements of a cocaine-binding aptamer by a thermodynamic and mutation study.

Thermodynamic and NMR analysis of inhibitor binding to dihydrofolate reductase.

Finding the path in an RNA folding landscape.

Engineering a structure switching mechanism into a steroid-binding aptamer and hydrodynamic analysis of the ligand binding mechanism.

Kinetic capillary electrophoresis with mass-spectrometry detection (KCE-MS) facilitates label-free solution-based kinetic analysis of protein-small molecule binding.

Quantitative affinity electrophoresis of RNA-small molecule interactions by cross-linking the ligand to acrylamide.

Quinine binding by the cocaine-binding aptamer. Thermodynamic and hydrodynamic analysis of high-affinity binding of an off-target ligand.

Kinetic size-exclusion chromatography with mass spectrometry detection: an approach for solution-based label-free kinetic analysis of protein-small molecule interactions.

Pre-equilibration kinetic size-exclusion chromatography with mass spectrometry detection (peKSEC-MS) for label-free solution-based kinetic analysis of protein-small molecule interactions.

Structure-affinity relationship of the cocaine-binding aptamer with quinine derivatives.

Salt-mediated two-site ligand binding by the cocaine-binding aptamer.

Comparison of the free and ligand-bound imino hydrogen exchange rates for the cocaine-binding aptamer.

Development of a thermal-stable structure-switching cocaine-binding aptamer.

Optimizing Stem Length To Improve Ligand Selectivity in a Structure-Switching Cocaine-Binding Aptamer.