electrophysiological-assessment-murine-atria-with-high-resolution

Electrophysiological Assessment of Murine Atria with High-Resolution Optical Mapping

Recent genome-wide association studies targeting atrial fibrillation (AF) have indicated a strong association between the genotype and ...

Tokyo Medical and Dental University

Usefulness of multimodality imaging on detecting plaque rupture in septal myocardial infarction associated with right bundle branch block.

Edema index measured by bioelectrical impedance analysis as a predictor of fluid reduction needed to remove clinical congestion in acute heart failure.

Venous congestion has come into focus as an important hemodynamic factor for worsening renal function (WRF) in patients with acute decompensated heart failure (ADHF). Serum alkaline phosphatase (ALP) was reported as a biological marker of liver congestion in ADHF. The purpose of this study was to determine whether ALP is a predictor of WRF in patients with ADHF.

Serum alkaline phosphatase as a predictor of worsening renal function in patients with acute decompensated heart failure.

The association between insulin resistance (IR) and coronary artery calcification (CAC) has been uncertain after adjustment for metabolic syndrome components. We aimed to evaluate whether IR is associated with CAC prevalence or progression independently of metabolic syndrome components.

Relationship of Insulin Resistance to Prevalence and Progression of Coronary Artery Calcification Beyond Metabolic Syndrome Components: Shiga Epidemiological Study of Subclinical Atherosclerosis.

Long-Term Prognosis of Catecholaminergic Polymorphic Ventricular Tachycardia Patients With Ryanodine Receptor (RYR2) Mutations.

Recent experimental studies have demonstrated that several microRNAs (miRNAs) expressed in atrial tissue promote a substrate of atrial fibrillation (AF). However, because it has not been fully elucidated whether these experimental data contribute to identifying circulating miRNAs as biomarkers for AF, we used a combined analysis of human serum and murine atrial samples with the aim of identifying these biomarkers for predicting AF.Methods and Results:Comprehensive analyses were performed to screen 733 miRNAs in serum from 10 AF patients and 5 controls, and 672 miRNAs in atrial tissue from 6 inducible atrial tachycardia model mice and 3 controls. We selected miRNAs for which expression was detected in both analyses, and their expression levels were changed in the human analyses, the murine analyses, or both. This screening identified 11 candidate miRNAs. Next, we quantified the selected miRNAs using a quantitative RT-PCR in 50 AF and 50 non-AF subjects. The individual assessment revealed that 4 miRNAs (miR-99a-5p, miR-192-5p, miR-214-3p, and miR-342-5p) were significantly upregulated in AF patients. A receiver-operating characteristics curve indicated that miR-214-3p and miR-342-5p had the highest accuracy. The combination of the 4 miRNAs modestly improved the predictive accuracy for AF (76% sensitivity, 80% specificity).

Masahiro Yamazoe

Department of Bio-informational Pharmacology,
Medical Research Institute,
Department of Bio-informational Pharmacology, Medical Research Institute

Tokyo Medical and Dental University

Electrophysiological Assessment of Murine Atria with High-Resolution Optical Mapping

Masahiro Yamazoe

.css-o250i3{font-size:18px;font-family:Open Sans,sans-serif;line-height:21.6px;}Department of Bio-informational Pharmacology,Medical Research Institute,Department of Bio-informational Pharmacology, Medical Research Institute

Tokyo Medical and Dental University

Electrophysiological Assessment of Murine Atria with High-Resolution Optical Mapping

Department of Bio-informational Pharmacology,
Medical Research Institute,
Department of Bio-informational Pharmacology, Medical Research Institute