automation-bio-atomic-force-microscope-measurements-on-hundreds-c

Automation of Bio-Atomic Force Microscope Measurements on Hundreds of <em>C. albicans</em> Cells

The method presented in this paper aims to automate Bio-AFM experiments and the recording of force curves. Using this method, it is possible to record ...

ENCB, Instituto Politécnico Nacional (IPN), Mexico City, Mexico

CIC, Instituto Politécnico Nacional (IPN), Mexico City, Mexico

Micropatterning and manipulation of mammalian and bacterial cells are important in biomedical studies to perform in vitro assays and to evaluate biochemical processes accurately, establishing the basis for implementing biomedical microelectromechanical systems (bioMEMS), point-of-care (POC) devices, or organs-on-chips (OOC), which impact on neurological, oncological, dermatologic, or tissue engineering issues as part of personalized medicine. Cell patterning represents a crucial step in fundamental and applied biological studies in vitro, hence today there are a myriad of materials and techniques that allow one to immobilize and manipulate cells, imitating the 3D in vivo milieu. This review focuses on current physical cell patterning, plus chemical and a combination of them both that utilizes different materials and cutting-edge micro-nanofabrication methodologies.

Adrian Martinez-Rivas

ENCB, Instituto Politécnico Nacional (IPN), Mexico City, Mexico

Automation of Bio-Atomic Force Microscope Measurements on Hundreds of C. albicans Cells