Department of Comparative Biology and Experimental Medicine
Dr. Ina Dobrinski is Professor of Reproductive Biology in the Faculty of Veterinary Medicine and the Cumming School of Medicine at the University of Calgary. She joined the University of Calgary in 2008 after 11 years at the University of Pennsylvania, where she was a Professor of Reproduction, the Director of the Center for Animal Transgenesis and Germ Cell Research, and the Marion Dilley and Robert George Jones Chair in Reproduction.
Research in the Dobrinski laboratory is focused on mammalian germline stem cell biology. This lab was the first to establish germ cell transplantation in non-rodent models to transmit a genetic change introduced into germline stem cells to the next generation. The work enables generation of germline gene-edited non-rodent animal models. They also developed xenografting of testis tissue and cells as an accessible in vivo system to study germline stem cells and spermatogenesis. These approaches allow controlled experimentation in donor species such as primates where experiments in whole animals would be logistically and ethically difficult.
More recently, her group pioneered testicular organoid technology as a reproducible in vitro model for cell-cell interactions in the mammalian testis.
Germ cell transplantation in pigs.
Biology of reproduction Jan, 2002 | Pubmed ID: 11751259
Sperm from neonatal mammalian testes grafted in mice.
Nature Aug, 2002 | Pubmed ID: 12181567
Germ cell transplantation in goats.
Molecular reproduction and development Apr, 2003 | Pubmed ID: 12589654
Progeny from sperm obtained after ectopic grafting of neonatal mouse testes.
Biology of reproduction Jun, 2003 | Pubmed ID: 12606381
Fertility and germline transmission of donor haplotype following germ cell transplantation in immunocompetent goats.
Biology of reproduction Oct, 2003 | Pubmed ID: 12801978
Accelerated maturation of primate testis by xenografting into mice.
Biology of reproduction May, 2004 | Pubmed ID: 14736818
Bovine sperm capacitation: assessment of phosphodiesterase activity and intracellular alkalinization on capacitation-associated protein tyrosine phosphorylation.
Molecular reproduction and development Apr, 2004 | Pubmed ID: 14991741
Thyroid function and pregnancy status in broodmares.
Journal of the American Veterinary Medical Association Mar, 2004 | Pubmed ID: 15070060
A game of cat and mouse: xenografting of testis tissue from domestic kittens results in complete cat spermatogenesis in a mouse host.
Journal of andrology Nov-Dec, 2004 | Pubmed ID: 15477365
Germ cell transplantation and testis tissue xenografting in domestic animals.
Animal reproduction science Oct, 2005 | Pubmed ID: 16055282
Germ cell transplantation.
Seminars in reproductive medicine Aug, 2005 | Pubmed ID: 16059832
Depletion of endogenous germ cells in male pigs and goats in preparation for germ cell transplantation.
Journal of andrology Nov-Dec, 2005 | Pubmed ID: 16291964
Recipient preparation and mixed germ cell isolation for spermatogonial stem cell transplantation in domestic cats.
Journal of andrology Mar-Apr, 2006 | Pubmed ID: 16304210
Germ cell fate and seminiferous tubule development in bovine testis xenografts.
Reproduction (Cambridge, England) Dec, 2005 | Pubmed ID: 16322552
Effects of 2-hydroxypropyl-beta-cyclodextrin and cholesterol on porcine sperm viability and capacitation status following cold shock or incubation.
Molecular reproduction and development May, 2006 | Pubmed ID: 16450405
Advances and applications of germ cell transplantation.
Human fertility (Cambridge, England) Mar, 2006 | Pubmed ID: 16581717
The length of the spermatogenic cycle is conserved in porcine and ovine testis xenografts.
Journal of andrology Jul-Aug, 2006 | Pubmed ID: 16598031
Protein gene product 9.5 is a spermatogonia-specific marker in the pig testis: application to enrichment and culture of porcine spermatogonia.
Molecular reproduction and development Dec, 2006 | Pubmed ID: 16894537
Building a testis: formation of functional testis tissue after transplantation of isolated porcine (Sus scrofa) testis cells.
Biology of reproduction Jan, 2007 | Pubmed ID: 17005941
Successful transplantation of bovine testicular cells to heterologous recipients.
Reproduction (Cambridge, England) Oct, 2006 | Pubmed ID: 17008473
Lsh is required for meiotic chromosome synapsis and retrotransposon silencing in female germ cells.
Nature cell biology Dec, 2006 | Pubmed ID: 17115026
Comparison of global gene expression between porcine testis tissue xenografts and porcine testis in situ.
Molecular reproduction and development Jun, 2007 | Pubmed ID: 17133601
Xenografting of adult mammalian testis tissue.
Animal reproduction science Jun, 2008 | Pubmed ID: 17512146
Transplantation of germ cells and testis tissue for the study and preservation of fertility.
Society of Reproduction and Fertility supplement , 2007 | Pubmed ID: 17644983
Adeno-associated virus (AAV)-mediated transduction of male germ line stem cells results in transgene transmission after germ cell transplantation.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology Feb, 2008 | Pubmed ID: 17873102
Ectopic grafting of mammalian testis tissue into mouse hosts.
Methods in molecular biology (Clifton, N.J.) , 2008 | Pubmed ID: 18370057
Xenografting of sheep testis tissue and isolated cells as a model for preservation of genetic material from endangered ungulates.
Reproduction (Cambridge, England) Jul, 2008 | Pubmed ID: 18390693
Maturation of testicular tissue from infant monkeys after xenografting into mice.
Endocrinology Oct, 2008 | Pubmed ID: 18566126
Cryopreservation of boar semen and its future importance to the industry.
Theriogenology Nov, 2008 | Pubmed ID: 18653225
Production of donor-derived sperm after spermatogonial stem cell transplantation in the dog.
Reproduction (Cambridge, England) Dec, 2008 | Pubmed ID: 18768666
Intratubular transplantation as a strategy for establishing animal models of testicular germ cell tumours.
International journal of experimental pathology Oct, 2008 | Pubmed ID: 18808526
Porcine embryos produced after intracytoplasmic sperm injection using xenogeneic pig sperm from neonatal testis tissue grafted in mice.
Reproduction, fertility, and development , 2008 | Pubmed ID: 18842182
De novo morphogenesis of functional testis tissue after ectopic transplantation of isolated cells.
Organogenesis Oct, 2007 | Pubmed ID: 19279705
Recent developments in testis tissue xenografting.
Reproduction (Cambridge, England) Aug, 2009 | Pubmed ID: 19372227
Asymmetric distribution of UCH-L1 in spermatogonia is associated with maintenance and differentiation of spermatogonial stem cells.
Journal of cellular physiology Aug, 2009 | Pubmed ID: 19388011
Isolation, characterization, and culture of human spermatogonia.
Biology of reproduction Feb, 2010 | Pubmed ID: 19846602
Expression pattern of acetylated alpha-tubulin in porcine spermatogonia.
Molecular reproduction and development Apr, 2010 | Pubmed ID: 20043318
Postnatal somatic cell proliferation and seminiferous tubule maturation in pigs: a non-random event.
Theriogenology Jul, 2010 | Pubmed ID: 20189235
Xenografting restores spermatogenesis to cryptorchid testicular tissue but does not rescue the phenotype of idiopathic testicular degeneration in the horse (Equus caballus).
Reproduction, fertility, and development , 2010 | Pubmed ID: 20353727
Development of bovine fetal testis tissue after ectopic xenografting in mice.
Journal of andrology May-Jun, 2011 | Pubmed ID: 20930193
Lymphoid-specific helicase (HELLS) is essential for meiotic progression in mouse spermatocytes.
Biology of reproduction Jun, 2011 | Pubmed ID: 21349825
Isolation of human male germ-line stem cells using enzymatic digestion and magnetic-activated cell sorting.
Methods in molecular biology (Clifton, N.J.) , 2012 | Pubmed ID: 22144235
Identification of spermatogonia by labeling for UCH-L1 in whole mounted caprine seminiferous tubules.
Molecular reproduction and development Dec, 2011 | Pubmed ID: 22231703
BNC1 is required for maintaining mouse spermatogenesis.
Genesis (New York, N.Y. : 2000) Jan, 2012 | Pubmed ID: 22266914
Characterization of the porcine testis-expressed gene 11 (Tex11).
Spermatogenesis 4, 2011 | Pubmed ID: 22319663
Suppression of spermatogenesis before grafting increases survival and supports resurgence of spermatogenesis in adult mouse testis.
Fertility and sterility Jun, 2012 | Pubmed ID: 22464084
Endocrine modulation of the recipient environment affects development of bovine testis tissue ectopically grafted in mice.
Reproduction (Cambridge, England) Jul, 2012 | Pubmed ID: 22550313
From in vitro culture to in vivo models to study testis development and spermatogenesis.
Cell and tissue research Sep, 2012 | Pubmed ID: 22842771
Testicular tissue grafting and male germ cell transplantation.
Methods in molecular biology (Clifton, N.J.) , 2013 | Pubmed ID: 22992939
Targeting of CRMP-2 to the primary cilium is modulated by GSK-3β.
PloS one , 2012 | Pubmed ID: 23185275
Viral transduction of male germline stem cells results in transgene transmission after germ cell transplantation in pigs.
Biology of reproduction Jan, 2013 | Pubmed ID: 23221397
Germ cell survival and differentiation after xenotransplantation of testis tissue from three endangered species: Iberian lynx (Lynx pardinus), Cuvier's gazelle (Gazella cuvieri) and Mohor gazelle (G. dama mhorr).
Reproduction, fertility, and development , 2014 | Pubmed ID: 23763851
MiRNA-20 and mirna-106a regulate spermatogonial stem cell renewal at the post-transcriptional level via targeting STAT3 and Ccnd1.
Stem cells (Dayton, Ohio) Oct, 2013 | Pubmed ID: 23836497
Goat embryonic stem-like cell derivation and characterization.
Methods in molecular biology (Clifton, N.J.) , 2013 | Pubmed ID: 23975805
De novo morphogenesis of testis tissue: an improved bioassay to investigate the role of VEGF165 during testis formation.
Reproduction (Cambridge, England) Jul, 2014 | Pubmed ID: 24803491
Primary cilia in the developing pig testis.
Cell and tissue research Nov, 2014 | Pubmed ID: 25107611
Xenografting of testicular tissue pieces: 12 years of an in vivo spermatogenesis system.
Reproduction (Cambridge, England) Nov, 2014 | Pubmed ID: 25150043
Phthalate esters affect maturation and function of primate testis tissue ectopically grafted in mice.
Molecular and cellular endocrinology Dec, 2014 | Pubmed ID: 25450860
Stirred suspension bioreactors as a novel method to enrich germ cells from pre-pubertal pig testis.
Andrology May, 2015 | Pubmed ID: 25877677
Beyond the mouse monopoly: studying the male germ line in domestic animal models.
ILAR journal , 2015 | Pubmed ID: 25991701
Use of Stirred Suspension Bioreactors for Male Germ Cell Enrichment.
Methods in molecular biology (Clifton, N.J.) , 2016 | Pubmed ID: 27066790
Primary cilia on porcine testicular somatic cells and their role in hedgehog signaling and tubular morphogenesis in vitro.
Cell and tissue research 04, 2017 | Pubmed ID: 27841005
TALEN-mediated gene targeting in porcine spermatogonia.
Molecular reproduction and development 03, 2018 | Pubmed ID: 29393557
Exposure to phthalate esters induces an autophagic response in male germ cells.
Environmental epigenetics Jul, 2017 | Pubmed ID: 29492312
Autologous grafting of cryopreserved prepubertal rhesus testis produces sperm and offspring.
Science (New York, N.Y.) 03, 2019 | Pubmed ID: 30898927
Formation of organotypic testicular organoids in microwell culture†.
Biology of reproduction Jun, 2019 | Pubmed ID: 30927418
Stirred Suspension Bioreactor Culture of Porcine Induced Pluripotent Stem Cells.
Stem cells and development Sep, 2019 | Pubmed ID: 31264514
Three-dimensional testicular organoids as novel models of testicular biology and toxicology.
Environmental epigenetics Jul, 2019 | Pubmed ID: 31463083
关于 JoVE
版权所属 © 2024 MyJoVE 公司版权所有,本公司不涉及任何医疗业务和医疗服务。