Francisca C. Bronfman

Department of Physiology, Faculty of Biological Sciences

Pontificia Universidad Católica de Chile

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Pontificia Universidad Católica de Chile

Universidad Andrés Bello

JOVE ARTICLES

An Improved Protocol to Purify and Directly Mono-Biotinylate Recombinant BDNF in a Tube for Cellular Trafficking Studies in Neurons

Nicolás Stuardo^1,2,

Guillermo Moya-Alvarado^1,3,

Carolina Ramírez^1,3,

Giampietro Schiavo⁴,

Francisca C. Bronfman^1,3

¹Department of Physiology, Faculty of Biological Sciences, Pontificia Universidad Católica de Chile,

²Department of Molecular and Cellular Biology, Faculty of Biological Sciences, Center for Aging and Regeneration (CARE UC), Pontificia Universidad Católica de Chile,

³Institute of Biomedical Sciences. Faculty of Medicine and Faculty of Life Sciences, Universidad Andrés Bello,

⁴Department of Neuromuscular Diseases, UCL Queen Square Institute of Neurology and UK Dementia Research Institute at UCL, University College London Campus

Neuroscience

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Francisca C. Bronfman

.css-o250i3{font-size:18px;font-family:Open Sans,sans-serif;line-height:21.6px;}Department of Physiology, Faculty of Biological Sciences

Pontificia Universidad Católica de Chile

An Improved Protocol to Purify and Directly Mono-Biotinylate Recombinant BDNF in a Tube for Cellular Trafficking Studies in Neurons

Department of Physiology, Faculty of Biological Sciences