multibac-system-based-purification-biophysical-characterization-human

MultiBac System-Based Purification and Biophysical Characterization of Human Myosin-7a

Myosin-7a is an actin-based motor protein vital for auditory and visual processes. Mutations in myosin-7a lead to Usher syndrome type 1, the most common ...

West Virginia University

Trees continuously regulate leaf physiology to acquire CO while simultaneously avoiding excessive water loss. The balance between these two processes, or water use efficiency (WUE), is fundamentally important to understanding changes in carbon uptake and transpiration from the leaf to the globe under environmental change. While increasing atmospheric CO (iCO ) is known to increase tree intrinsic water use efficiency (iWUE), less clear are the additional impacts of climate and acidic air pollution and how they vary by tree species. Here, we couple annually resolved long-term records of tree-ring carbon isotope signatures with leaf physiological measurements of Quercus rubra (Quru) and Liriodendron tulipifera (Litu) at four study locations spanning nearly 100 km in the eastern United States to reconstruct historical iWUE, net photosynthesis (A ), and stomatal conductance to water (g ) since 1940. We first show 16%-25% increases in tree iWUE since the mid-20th century, primarily driven by iCO , but also document the individual and interactive effects of nitrogen (NO ) and sulfur (SO ) air pollution overwhelming climate. We find evidence for Quru leaf gas exchange being less tightly regulated than Litu through an analysis of isotope-derived leaf internal CO (C ), particularly in wetter, recent years. Modeled estimates of seasonally integrated A and g revealed a 43%-50% stimulation of A was responsible for increasing iWUE in both tree species throughout 79%-86% of the chronologies with reductions in g attributable to the remaining 14%-21%, building upon a growing body of literature documenting stimulated A overwhelming reductions in g as a primary mechanism of increasing iWUE of trees. Finally, our results underscore the importance of considering air pollution, which remains a major environmental issue in many areas of the world, alongside climate in the interpretation of leaf physiology derived from tree rings.

Differences in leaf gas exchange strategies explain Quercus rubra and Liriodendron tulipifera intrinsic water use efficiency responses to air pollution and climate change.

Marvin Wright

Department of Biochemistry & Molecular Medicine,
School of Medicine,
Department of Biochemistry & Molecular Medicine, School of Medicine

West Virginia University

MultiBac System-Based Purification and Biophysical Characterization of Human Myosin-7a

Marvin Wright

Department of Biochemistry & Molecular Medicine,School of Medicine,Department of Biochemistry & Molecular Medicine, School of Medicine

West Virginia University

MultiBac System-Based Purification and Biophysical Characterization of Human Myosin-7a

Department of Biochemistry & Molecular Medicine,
School of Medicine,
Department of Biochemistry & Molecular Medicine, School of Medicine