concurrent-quantification-cellular-extracellular-components

Concurrent Quantification of Cellular and Extracellular Components of Biofilms

Confocal laser scanning microscopy (CLSM) is a powerful tool for investigation of biofilms. Very few investigations have successfully quantified ...

University of Oklahoma Health Sciences Center

The aim of this study was to assess the effect on microleakage of cyclic lateral fatigue forces in different types of cervical resin composite restorations.

Effect of cyclical lateral forces on microleakage in cervical resin composite restorations.

A radiographic recall evaluation of 894 endodontic cases treated by dental students at the University of Oklahoma College of Dentistry was conducted by faculty. The purpose of this study was to determine the success of standardized nonsurgical endodontic treatments by using radiographic techniques of determination and to compare the results to similar studies at other dental schools. Cases were labeled as successful, acceptable, questionable, and failing. Observations included time since treatment, age, gender, and tooth type. The combined percentage of successful and acceptable cases was 91.05%. Differences in percentage of success among recall time intervals were statistically significant (p < 0.01), as were the percentages of success between tooth types treated (p < 0.05). Success rate was not affected by age or gender (p > 0.05). The overall rate of successful and acceptable cases in this study was found to compare favorably with those from recall studies conducted at other dental schools.

A radiographic recall evaluation of 894 endodontic cases treated in a dental school setting.

This investigation measured the deactivation (unloading) force-deflection behavior of selected multistranded stainless steel orthodontic wires. The guidelines from the revised American National Standards Institute/American Dental Association Specification No. 32 for orthodontic wires (type 2, nonlinear elasticity) were used to perform a 3-point bending test to obtain data for the deactivation force levels at 3, 2, 1, and 0.5 mm, and permanent deformation values. The study tested 20 wire designs: triple-stranded twisted wires, 5- and 6-stranded coaxial wires, and 8- and 9-stranded braided rectangular wires of selected cross-sections. Twenty specimens were tested for each wire design. The specification guidelines for testing provided a means for obtaining comparative unloading force delivery data for the deflections tested. Multistranded wires of significantly different cross-section, weave, and strand number fell within relatively narrow ranges of force delivery at the 0.5-mm (1.7-42.7 grams-force [gmf]) and 1-mm (31.1-134.2 gmf) deflections and wider ranges of force levels at the 2-mm (57.0-320.5 gmf) and 3-mm (72.5-451.8 gmf) deflections. All wires exhibited some permanent deformation, but none averaged greater than 0.66 mm. The testing also showed that multistranded wires of significantly different design exhibit similar force levels. The guidelines of the revised Specification No. 32 yielded deactivation data with regard to force delivery and provided comparisons of the wire designs tested.

Deactivation force-deflection behavior of multistranded stainless steel wires.

This study compared the degree of microleakage in the proximal walls of direct and indirect resin slot restorations in relation to the types of dentin bonding systems and the location of gingival margins. Two Class II slot preparations were prepared and restored in each of 60 extracted human molars using direct (Filtek Supreme) and indirect (Tescera ATL) restorative resin materials. Various types of dentin bonding systems, including self-etching (OneStep Plus/Tyrian SPE, iBond, Xeno III) and etch and rinse systems (All-Bond 2, Prime & Bond NT) were used to restore the prepared teeth. The gingival proximal wall was placed apical to the cementoenamel junction (CEJ) in 1 proximal box and coronal to the CEJ in the other. The specimens were stained and evaluated for microleakage using a digital imaging and analysis system. Significant differences were found in the degree of microleakage observed in the various restorative groups. In general, the group restored with indirect resin had less microleakage than the direct resin groups. Factors, such as type of dentin bonding system and location of gingival margins, exert a substantial influence on the degree of microleakage that occurred along the walls of proximal resin restorations.

Microleakage in the proximal walls of direct and indirect posterior resin slot restorations.

Chitosan is a well sought-after polysaccharide in biomedical applications due to its biocompatibility, biodegradability to non-toxic substances, and ease of fabrication into various configurations. However, alterations in the anti-bacterial properties of chitosan in various forms is not completely understood. The objective of this study was to evaluate the anti-bacterial properties of chitosan matrices in different configurations against two pathogens-Gram-positive Streptococcus mutans and Gram-negative Actinobacillus actinomycetemcomitans. Two-dimensional (2-D) membranes and three-dimensional (3-D) porous scaffolds were synthesized by air drying and controlled-rate freeze drying. Matrices were suspended in bacterial broths with or without lysozyme (enzyme that degrades chitosan). Influences of pore size, blending with Polycaprolactone (PCL, a synthetic polymer), and neutralization process on bacterial proliferation were studied. Transient changes in optical density of the broth, adhesion characteristics, viability, and contact-dependent bacterial activity were assessed. 3-D porous scaffolds were more effective in reducing the proliferation of S. mutans in suspension than 2-D membranes. However, no significant differences were observed on the proliferation of A. actinomycetemcomitans. Presence of lysozyme significantly increased the antibacterial activity of chitosan against A. actinomycetemcomitans. Pore size did not affect the proliferation kinetics of either species, with or without lysozyme. NaOH neutralization of chitosan increased bacterial adhesion whereas ethanol neutralization inhibited adhesion without lowering proliferation. Mat culture tests indicated that chitosan does not allow proliferation on its surface and it loses antibacterial activity upon blending with PCL. Results suggest that the chemical and structural characteristics of chitosan-based matrices can be manipulated to influence the interaction of different bacterial species.

Antibacterial activity of chitosan-based matrices on oral pathogens.

Globalization is a broad term referring to the increasing connectivity, integration, and interdependence of economies, societies, technologies, cultures, and political and ecological spheres across the world. This position paper was developed by a working group of the 2007 American Dental Education Association (ADEA) Leadership Institute. The authors explore the effect that globalization has had on dentistry and dental education to date and hypothesize what dental education could look like in the years ahead. While the paper is written from a North American perspective, some of the authors bring international expertise and experience to the topic of global dental education in a flat world. Specific issues and barriers addressed in this position paper include variations in accreditation and licensure requirements in dental education throughout the world; the historical development of dental education models (odontology and stomatology) and the need for congruency of these models in the global environment; the competency-based model of education and its relevance to development and implementation of global dental competencies; and the slow adoption of technological advances in dental education for promoting collaborations and encouraging resource sharing among countries. These challenges are discussed as they affect the implementation of a standardized global dental education that can lead to improved access to oral health care services and better oral and overall health for the citizens of the world.

Sharukh S. Khajotia

Department of Dental Materials,
College of Dentistry,
Department of Dental Materials, College of Dentistry

University of Oklahoma Health Sciences Center

Concurrent Quantification of Cellular and Extracellular Components of Biofilms

Sharukh S. Khajotia

.css-o250i3{font-size:18px;font-family:Open Sans,sans-serif;line-height:21.6px;}Department of Dental Materials,College of Dentistry,Department of Dental Materials, College of Dentistry

University of Oklahoma Health Sciences Center

Concurrent Quantification of Cellular and Extracellular Components of Biofilms

Department of Dental Materials,
College of Dentistry,
Department of Dental Materials, College of Dentistry