creating-adhesive-soluble-gradients-for-imaging-cell-migration-with

Creating Adhesive and Soluble Gradients for Imaging Cell Migration with Fluorescence Microscopy

Cells can sense and migrate towards higher concentrations of adhesive cues such as the glycoproteins of the extracellular matrix and soluble cues such as ...

The University of New South Wales

Cell migration contributes to cancer metastasis and involves cell adhesion to the extracellular matrix (ECM), force generation through the cell's cytoskeletal, and finally cell detachment. Both adhesive cues from the ECM and soluble cues from neighbouring cells and tissue trigger intracellular signalling pathways that are essential for cell migration. While the machinery of many signalling pathways is relatively well understood, how hierarchies of different and conflicting signals are established is a new area of cellular cancer research. We examine the recent advances in microfabrication, microfluidics, and nanotechnology that can be utilized to engineer micro- and nanoscaled cellular environments. Controlling both adhesive and soluble cues for migration may allow us to decipher how cells become motile, choose the direction for migration, and how oncogenic transformations influences these decision-making processes.

How do cells make decisions: engineering micro- and nanoenvironments for cell migration.

Migration cellulaire contribue &agrave; m&eacute;tastases canc&eacute;reuses et implique l&#39;adh&eacute;sion cellulaire &agrave; la matrice extracellulaire (ECM), mise sur pied par l&#39;interm&eacute;diaire de la cellule du cytosquelette et enfin le d&eacute;tachement cellulaire. Les adh&eacute;sif cues de l&#39;ECM et d&eacute;clenchent des rep&egrave;res solubles &agrave; partir de cellules voisines et les tissus des voies de signalisation intracellulaires qui sont essentiels pour la migration cellulaire. Alors que les machines de nombreuses voies de signalisation sont relativement bien compris, comment sont &eacute;tablis les hi&eacute;rarchies des signaux diff&eacute;rents et contradictoires sont un nouveau domaine de recherche sur le cancer cellulaire. Nous examinons les progr&egrave;s r&eacute;cents dans la microfabrication, microfluidique et nanotechnologie qui peut &ecirc;tre utilis&eacute;e pour la micro - et minimisations des environnements cellulaires de l&#39;ing&eacute;nieur. Contr&ocirc;ler les rep&egrave;res adh&eacute;sifs et solubles migration peut-&ecirc;tre nous permettent de d&eacute;chiffrer comment les cellules deviennent motiles, choisissez la direction pour les migrations, et comment des transformations oncog&egrave;nes influe sur ces processus d&eacute;cisionnels.

Comment les cellules prennent des d&eacute;cisions : ing&eacute;nierie des micro - et nanoenvironments pour la migration cellulaire.

Migrazione delle cellule contribuisce a metastasi del cancro e implica l&#39;adesione delle cellule alla matrice extracellulare (ECM), generazione di forza attraverso la cella del citoscheletro e infine distacco delle cellule. Adesivo spunti da ECM sia solubili spunti da cellule vicine e tessuto innescano percorsi di segnalazione intracellulare che sono essenziali per la migrazione delle cellule. Mentre il macchinario di molte vie di segnalazione &egrave; ben nota, come vengono stabilite le gerarchie di segnali differenti e contrastanti &egrave; una nuova area di ricerca sul cancro cellulare. Esaminiamo i recenti progressi nella microfabbricazione, microfluidica e nanotecnologia che pu&ograve; essere utilizzata per progettare ambienti cellulari micro - e nanoparticelle. Controllo spunti solubili e adesivi per migrazione pu&ograve; permetterci di decifrare come cellule diventano motili, scegliere la direzione per la migrazione, e come trasformazione oncogenica influenze questi processi decisionali.

Come fanno le cellule le decisioni: ingegneria micro - e nanoenvironments per la migrazione delle cellule.

Migraci&oacute;n celular contribuye a la met&aacute;stasis del c&aacute;ncer e implica la adhesi&oacute;n celular a la matriz extracelular (MEC), generaci&oacute;n de fuerza a trav&eacute;s de la c&eacute;lula citoesqueleto y finalmente la separaci&oacute;n de la c&eacute;lula. Adhesivo de se&ntilde;ales del ECM tanto se&ntilde;ales solubles de tejido y c&eacute;lulas vecinas activan v&iacute;as de se&ntilde;alizaci&oacute;n intracelulares que son esenciales para la migraci&oacute;n celular. Mientras que la maquinaria de muchas v&iacute;as de se&ntilde;alizaci&oacute;n se entiende relativamente bien, c&oacute;mo se establecen jerarqu&iacute;as de se&ntilde;ales diferentes y contradictorias es una nueva &aacute;rea de investigaci&oacute;n del c&aacute;ncer celular. Examinamos los avances recientes en la microfabricaci&oacute;n, microflu&iacute;dica y nanotecnolog&iacute;a que puede ser utilizado para dise&ntilde;ar ambientes celulares micro y nanoscaled. Controlar se&ntilde;ales adhesivas y solubles para migraci&oacute;n puede permitir descifrar c&oacute;mo las c&eacute;lulas se convierten en m&oacute;viles, elegir la direcci&oacute;n de migraci&oacute;n, y c&oacute;mo las transformaciones oncog&eacute;nicas influye en estos procesos de toma de decisiones.

&iquest;C&oacute;mo las c&eacute;lulas decisiones: Ingenier&iacute;a micro y nanoenvironments para la migraci&oacute;n celular.

Smart surfaces presenting both antifouling molecules with a charged functional group at their distal end, and molecules that are terminated by RGD peptides for cell adhesion, were fabricated and characterized (see picture). By applying potentials of +300 or -300â€…mV, the surfaces could be dynamically switched to make the peptide accessible or inaccessible to cells.

Using an electrical potential to reversibly switch surfaces between two states for dynamically controlling cell adhesion.

Surfaces intelligentes pr&eacute;sentant les mol&eacute;cules antifouling avec un groupe fonctionnel charg&eacute; &agrave; leur extr&eacute;mit&eacute; distale et des mol&eacute;cules qui sont terminent par des peptides RGD pour cell adhesion, ont &eacute;t&eacute; fabriqu&eacute;s et caract&eacute;ris&eacute;es (voir photo). En appliquant les potentiels de + 300 ou -300 mV, les surfaces peuvent &ecirc;tre commut&eacute;s dynamiquement pour rendre le peptide accessibles ou inaccessibles aux cellules.

D&#39;employer un potentiel &eacute;lectrique et r&eacute;versiblement surfaces entre deux &Eacute;tats pour contr&ocirc;ler dynamiquement adh&eacute;rence cellulaire.

Superfici intelligenti, presentando sia antivegetativa molecole con un caricato gruppo funzionale alla loro estremit&agrave; distale e le molecole che sono terminati da peptidi RGD per cell adesione, fabbricate e caratterizzate (vedi foto). Applicando le potenzialit&agrave; di 300 o -300 mV, le superfici potrebbero essere commutate in modo dinamico per rendere il peptide accessibile o inaccessibile alle cellule.

Siti Hawa Ngalim

Centre for Vascular Research and Australian Centre for Nanomedicine

The University of New South Wales

Creating Adhesive and Soluble Gradients for Imaging Cell Migration with Fluorescence Microscopy

Siti Hawa Ngalim

.css-o250i3{font-size:18px;font-family:Open Sans,sans-serif;line-height:21.6px;}Centre for Vascular Research and Australian Centre for Nanomedicine

The University of New South Wales

Creating Adhesive and Soluble Gradients for Imaging Cell Migration with Fluorescence Microscopy

Centre for Vascular Research and Australian Centre for Nanomedicine