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Creating Adhesive and Soluble Gradients for Imaging Cell Migration with Fluorescence Microscopy

DOI :

10.3791/50310-v

April 4th, 2013

April 4th, 2013

12,395 Views

1Centre for Vascular Research and Australian Centre for Nanomedicine, The University of New South Wales, 2School of Chemistry and Australian Centre for Nanomedicine, The University of New South Wales

A method for the assembly of adhesive and soluble gradients in a microscopy chamber for live cell migration studies is described. The engineered environment combines antifouling surfaces and adhesive tracks with solution gradients and therefore allows one to determine the relative importance of guidance cues.

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