Institut Curie, PSL Research University, CNRS UMR 3348, Orsay

1 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

Simultaneous Assessment of Kinship, Division Number, and Phenotype via Flow Cytometry for Hematopoietic Stem and Progenitor Cells

Alessandro Donada¹, Tamar Tak¹, Giulio Prevedello^1,2,3,4, Idan Milo¹, Ken R. Duffy², Leïla Perié¹

¹Quantitative Immuno-hematology, CNRS UMR168, Institut Curie, ²Hamilton Institute, Maynooth University, ³Institut Curie, PSL Research University, CNRS UMR 3348, Orsay, ⁴Université Paris-Sud, Université Paris-Saclay, CNRS UMR 3348, Orsay

Presented here is a flow cytometry-based technique that allows for simultaneously measuring the number of cell divisions, surface cell phenotype, and cellular kinship. Those properties can be tested statistically using a permutation-based framework.