Roche Innovation Centre Basel

1 ARTICLES PUBLISHED IN JoVE

Biology

A Simple, Quick, and Partially Automated Protocol for the Isolation of Single Nuclei from Frozen Mammalian Tissues for Single Nucleus Sequencing

Lucas Stalder¹, Fabian Koechl¹, Kerstin Hahn¹, Marc Sultan¹, Megana K. Prasad¹

¹Roche Pharma Research and Early Development, Pharmaceutical Sciences, Roche Innovation Centre Basel

The study describes a simple, quick, and partially automated protocol to isolate high-quality nuclei from frozen mammalian tissues for downstream single nuclei RNA sequencing.