High-Throughput Expression and Purification of Human Solute Carriers for Structural and Biochemical StudiesSagar Raturi 1, Huanyu Li 1, Yung-Ning Chang 2, Andreea Scacioc 1, Tina Bohstedt 1, Alejandra Fernandez-Cid 1, Adam Evans 1, Patrizia Abrusci 1, Abilasha Balakrishnan 1, Tomas C. Pascoa 1, Didi He 1, Gamma Chi 1, Nanki Kaur Singh 1, Mingda Ye 1, Anna Li 1, Leela Shrestha 1, Dong Wang 1, Eleanor P. Williams 1, Nicola A. Burgess-Brown 1, Katharina L. Dürr 1, Vera Puetter 2, Alvaro Ingles-Prieto 3, David B. Sauer 1
1Centre for Medicines Discovery, Nuffield Department of Medicine, University of Oxford, 2Nuvisan ICB GmbH, 3CeMM Research Center for Molecular Medicine of the Austrian Academy of Sciences
Structural and biochemical studies of human membrane transporters require milligram quantities of stable, intact, and homogeneous protein. Here we describe scalable methods to screen, express, and purify human solute carrier transporters using codon-optimized genes.
