National Institute of Biology
2 ARTICLES PUBLISHED IN JoVE
Biology
Studying Cell Death Initiation Using a Digital Microscope
Tina Arnšek *1, Nuša Golob *1, Nastja Marondini 1, Maruša Pompe-Novak 1,2, Kristina Gruden 1, Tjaša Lukan 1
1National Institute of Biology, 2University of Nova Gorica
Here we present a protocol for studying the rate of programmed cell death initiation by continuously imaging inoculated leaves following cell death induction.
Biology
High Throughput Image-Based Phenotyping for Determining Morphological and Physiological Responses to Single and Combined Stresses in Potato
Lamis Osama Anwar Abdelhakim 1, Barbora Pleskačová 1, Natalia Yaneth Rodriguez-Granados 2, Rashmi Sasidharan 2, Lucia Sandra Perez-Borroto 3, Sophia Sonnewald 4, Kristina Gruden 5, Ute C. Vothknecht 6, Markus Teige 7, Klára Panzarová 1
1PSI (Photon Systems Instruments), spol. s r.o. Drasov, 2Plant Stress Resilience, Institute of Environmental Biology, Utrecht University, 3Plant Breeding, Wageningen University and Research, 4Department of Biology, Biochemistry, Friedrich-Alexander Universität Erlangen-Nürnberg, 5Department of Biotechnology and Systems Biology, National Institute of Biology, 6Plant Cell Biology, Institute of Cellular and Molecular Botany, University of Bonn, 7Department of Functional & Evolutionary Ecology, University of Vienna
We designed an image-based phenotyping protocol to determine the morphological and physiological responses to single and combined heat, drought, and waterlogging treatments. This approach enabled the identification of early, late, and recovery responses at a whole plant level, particularly above-ground parts, and highlighted the necessity of using multiple imaging sensors.
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