Pseudotyped Viruses As a Molecular Tool to Monitor Humoral Immune Responses Against SARS-CoV-2 Via Neutralization AssayTobia Fantoni *1, Michele Bissoli *1, Chiara Stefani 1, Mauro Voi 1, Alexandrina Dabija 1, Rebecca Casula 1, Domenico Luca Minafra 1, Julys da Fonseca Palmeira 2, Enrique Roberto Argañaraz 2, Martin Mayora-Neto 3, Nigel J. Temperton 3, Donato Zipeto 1, Alessandra Ruggiero 1
1Department of Neuroscience, Biomedicine and Movement, University of Verona, 2Laboratory of Molecular Neurovirology, Faculty of Health Science, University of Brasília, 3Viral Pseudotype Unit, Medway School of Pharmacy, Universities of Kent and Greenwich at Medway
Pseudotyped viruses (PVs) are replication-defective virions that are used to study host-virus interactions under safer conditions than handling authentic viruses. Presented here is a detailed protocol that shows how SARS-CoV-2 PVs can be used to test the neutralizing ability of patients' serum after COVID-19 vaccination.
