Evaluating Toxicity of Chemicals using a Zebrafish Vibration Startle Response Screening SystemGaëlle Hayot 1, Daniel Marcato 1,2, Christina A. Cramer von Clausbruch 1, Giuseppina Pace 1, Uwe Strähle 1,3, John K. Colbourne 4, Christian Pylatiuk 5, Ravindra Peravali 1, Carsten Weiss 1, Stefan Scholz 6, Thomas Dickmeis 1
1Institute of Biological and Chemical Systems - Biological Information Processing, Karlsruhe Institute of Technology - Campus Nord, 2DITABIS AG - Digital Biomedical Imaging Systems AG, 3Centre for Organismal Studies, Heidelberg University, 4School of Biosciences, University of Birmingham, 5Institute for Automation and Applied Informatics, Karlsruhe Institute of Technology - Campus Nord, 6Department of Bioanalytical Ecotoxicology, Helmholtz-Centre for Environmental Research - UFZ
We describe a screening system's workflow and data analysis for evaluating chemical compound toxicity based on the zebrafish embryo vibration startle response. The system records the movements of zebrafish embryos upon exposure to a vibration stimulus and allows for an integrated evaluation of general toxicity/lethality and neuromuscular toxicity.
