SUSTech University

1 ARTICLES PUBLISHED IN JoVE

Developmental Biology

Visualizing Low-Abundance Proteins and Post-Translational Modifications in Living Drosophila Embryos via Fluorescent Antibody Injection

Qihong Zheng^*1, Xiaoxiang Xiang^*1, Yan Yan^2,3, Huapeng H. Yu¹

¹School of Life Sciences, SUSTech University, ²Shenzhen PKU-HKUST Medical Center, ³Division of Life Science, Hong Kong University of Science and Technology

This protocol describes the customized antibody-based fluorescence labeling and injection into early Drosophila embryos to enable live imaging of low-abundance proteins or post-translational modifications that are challenging to detect using traditional GFP/mCherry-tag approaches.