Henry M. Jackson Foundation
2 ARTICLES PUBLISHED IN JoVE
Neuroscience
A High Content Imaging Assay for Identification of Botulinum Neurotoxin Inhibitors
Krishna P. Kota 1,6, Veronica Soloveva 2,6,7, Laura M. Wanner 3,6, Glenn Gomba 4,6, Erkan Kiris 3,5,6, Rekha G. Panchal 6, Christopher D. Kane 2,6,7, Sina Bavari 6
1Perkin Elmer Inc., 2Henry M. Jackson Foundation, 3The Geneva Foundation, 4ORISE, 5Frederick National Laboratory for Cancer Research, 6Division of Molecular and Translational Sciences, US Army Medical Research Institute of Infectious Diseases, 7DoD Biotechnology High Performance Computing Software Applications Institute (BHSAI), Telemedicine and Advanced Technology Research Center (TATRC), US Army Medical Research and Materiel Command (USAMRMC)
Botulinum neurotoxin is one of the most potent toxins among Category-A biothreat agents, yet a post-exposure therapeutic is not available. The high content imaging approach is a powerful methodology for identifying novel inhibitors as it enables multiparameter screening using biologically relevant motor neurons, the primary target of this toxin.
Immunology and Infection
Pairwise Growth Competition Assay for Determining the Replication Fitness of Human Immunodeficiency Viruses
Siriphan Manocheewa 1, Erinn C. Lanxon-Cookson 1, Yi Liu 1, J. Victor Swain 1, Jan McClure 1, Ushnal Rao 1, Brandon Maust 1, Wenjie Deng 1, Justine E. Sunshine 1, Moon Kim 1, Morgane Rolland 3,4, James I. Mullins 1,2
1Department of Microbiology, University of Washington, 2Departments of Medicine and Laboratory Medicine, University of Washington, 3U.S Military HIV Research Program, Walter Reed Army Institute of Research, 4Henry M. Jackson Foundation
Growth competition between nearly isogenic viruses provides a sensitive measurement for determining relative replication fitness. The protocols described here include the construction of recombinant HIV-1 clones, virus propagation and growth competition and analysis methods optimized to yield sensitive and consistent results.
