Wu Han NO.1 Hospital

1 ARTICLES PUBLISHED IN JoVE

Cancer Research

Multiplex Cyclic Fluorescent Immunohistochemistry

Yun Chen^*1, Hui Zhang^*2, Yaodong Fan³, Liu Yang^4,5, Yan Dong^*1

¹Department of Pathology, The Third Affiliated Hospital of Kunming Medical University & Yunnan Cancer Hospital, ²Department of Gastroenterology, Wu Han NO.1 Hospital, ³Department of Neurosurgery, The Third Affiliated Hospital of Kunming Medical University & Yunnan Cancer Hospital, ⁴Departments of Biomedical Research Center, The Affiliated Calmette Hospital of Kunming Medical University & The First Hospital of Kunming, ⁵School of Medical, Yunnan University

Multiplex cyclic immunohistochemistry allows in situ detection of multiple markers simultaneously using repeated antigen-antibody incubation, image scanning, and image alignment and integration. Here, we present the operating protocol for identifying immune cell substrates with this technology in lung cancer and paired brain metastasis samples.