Fondazione Policlinico Universitario ‘A. Gemelli’ - IRCCS

1 ARTICLES PUBLISHED IN JoVE

Cancer Research

Co-Culture In Vitro Systems to Reproduce the Cancer-Immunity Cycle

Nicoletta Manduca^*1, Ester Maccafeo^*1, Adele De Ninno², Antonella Sistigu^1,3, Martina Musella¹

¹Dipartimento di Medicina e Chirurgia Traslazionale, Università Cattolica del Sacro Cuore, ²CNR Institute for Photonics and Nanotechnology, ³Fondazione Policlinico Universitario ‘A. Gemelli’ - IRCCS

Here, we detail a simple, fast, and reliable multiparametric flow cytometry- and tumor-on-a-chip-assisted protocol to monitor and characterize the steps constituting the cancer-immunity cycle. Indeed, a thorough understanding of the interplays between cancer and immune cells provides critical insights to outsmart tumors and guide clinical care.