SP Pune University Campus

1 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

Measuring Endoplasmic Reticulum Stress and Unfolded Protein Response in HIV-1 Infected T-Cells and Analyzing its Role in HIV-1 Replication

Anjali Tripathi¹, Anindita Dasgupta¹, Debashis Mitra¹

¹National Centre for Cell Science, SP Pune University Campus

Here, we describe some established methods to determine endoplasmic reticulum (ER) stress and unfolded protein response (UPR) activation, with particular emphasis on HIV-1 infection. This article also describes a set of protocols to investigate the effect of ER stress/UPR on HIV-1 replication and virion infectivity.