Shan Xi Medical University

1 ARTICLES PUBLISHED IN JoVE

Neuroscience

Handcrafted Silicone Coated Filament for Mice Middle Cerebral Artery Occlusion Models

Min Zhang^1,2, Yitong Yuan³, Ruochen Du³, Chunli Wen¹, Shiqin Lin², Yaqiong Zhang², Zhenyu Xiang⁴, Fengyun Hu¹, Chunfang Wang³

¹Department of Neurology, Fifth Hospital of Shanxi Medical University, Shanxi Provincial People's Hospital, ²Department of Neurology, Taiyuan Central Hospital, Shan Xi Medical University, ³Experimental Animal Center, Shanxi Medical University, ⁴Collaborative Innovation Center for Molecular Imaging of Precision Medicine, Shan Xi Medical University

This protocol describes a straightforward method for creating coated filaments for the middle cerebral artery occlusion (MCAO) model in mice using silicone, nylon sutures, and syringe needles. This method allows for the production of filaments with a consistent diameter and various silicone wrapping lengths tailored to experimental needs.