Isolation of Hypothalamic Microglia from Freshly Perfused Adult Mouse Brain by Magnetic-Activated Cell SortingEvangelia Kyriakidou *1, Giulia Cutugno *1, Camille Allard 1, Agnes Nadjar 1
1University of Bordeaux, INSERM, Neurocentre Magendie
We describe a protocol for isolating microglial cells from the mouse hypothalamus (or equivalent small brain structures) using magnetically activated cell sorting (MACS), in a relatively short time. The MACS-sorted hypothalamic microglia can be used for ex vivo analysis and can be plated to perform in vitro assays.
