Whole Blood Assay with Dual Co-Stimulation for Antigen-Specific Analysis of Host Immunity to Fungal and Viral PathogensLukas Page 1, Chris D. Lauruschkat 2, Kevin Dennehy 1, Eva Loell 1, Beeke Tappe 2, Andre Fuchs 3, Sebastian Wurster *4, Reinhard Hoffmann *1
1Institute for Laboratory Medicine and Microbiology, University Hospital Augsburg, 2Department of Internal Medicine II, University Hospital of Wuerzburg, 3Internal Medicine III - Gastroenterology and Infectious Diseases, University Hospital Augsburg, 4Department of Infectious Diseases, Infection Control and Employee Health, MD Anderson Cancer Center, University of Texas Houston
Whole blood-based immunoassays provide a facile and resource-efficient tool to analyze antigen-specific immunity for diagnostic and research purposes. This article provides an optimized whole blood-based protocol with dual co-stimulation for comprehensive analysis of host immunity to fungal and viral pathogens, including a low-volume version for pediatric patients and small animals.
