Longitudinal In Vivo Imaging of Retinal Cells with Tailored Adeno-Associated Virus Serotypes via Confocal Scanning Laser OphthalmoscopyTianmin Ren 1, Xiaowei Fan 1, Kailai Chen 2, Jiashuo Liu 2, Jingxue Zhang 1,3,4, Ningli Wang 1,3,4
1Beijing Institute of Ophthalmology, Beijing Tongren Eye Center, Beijing Tongren Hospital, Capital Medical University, Beijing Ophthalmology & Visual Sciences Key Laboratory, 2Second Clinical Medical School, Capital Medical University, 3Beijing Institute of Brain Disorders, Collaborative Innovation Center for Brain Disorders, Capital Medical University, 4Beijing Key Laboratory of Fundamental Research on Biomechanics in Clinical Application, Capital Medical University
This protocol describes the use of adeno-associated virus (AAV) vectors for cell-specific labeling and in vivo imaging using a confocal scanning laser ophthalmoscope (CSLO). This method enables the investigation of different retinal cell types and their contributions to retinal function and disease.
