Boston Biomedical Research Institute
2 ARTICLES PUBLISHED IN JoVE
Biology
Growth Assays to Assess Polyglutamine Toxicity in Yeast
Martin L. Duennwald 1
1Boston Biomedical Research Institute
This manuscript describes three complementary protocols for assessing the toxicity of polyglutamine (polyQ)-expansion proteins in the yeast Saccharomyces cerevisiae. These protocols can easily be modified to monitor the toxicity of other misfolded proteins in yeast.
Biology
Protease- and Acid-catalyzed Labeling Workflows Employing 18O-enriched Water
Diana Klingler 1, Markus Hardt 1
1Boston Biomedical Research Institute
Stable isotope labeling workflows employing 18O-enriched water (LeO-workflows) are versatile tools for quantitative and qualitative proteomics studies. In protease-assisted (PALeO) workflows, 18O-atoms are introduced by proteolytic cleavage and carboxyl oxygen exchange reactions mediated by proteases. In the acid-catalyzed (ALeO) workflow, 18O-atoms are introduced by carboxyl oxygen exchange at low pH.
