Ludwig-Maximilians-University

2 ARTICLES PUBLISHED IN JoVE

Medicine

Real-Time Electrocardiogram Monitoring During Treadmill Training in Mice

Philipp Tomsits^*1,2,4, Aparna Sharma Chivukula^*1,2, Kavi Raj Chataut^*1,2, Agus Simahendra³, Ludwig T. Weckbach^1,2,3, Stefan Brunner¹, Sebastian Clauss^1,2,4

¹Department of Medicine I, University Hospital Munich, Ludwig-Maximilians University Munich (LMU), ²DZHK (German Centre for Cardiovascular Research), Partner Site Munich, Munich Heart Alliance (MHA), ³Institute of Cardiovascular Physiology and Pathophysiology, Biomedical Center, Ludwig-Maximilians-University, ⁴Institute of Surgical Research at the Walter-Brendel-Centre of Experimental Medicine, University Hospital Munich, Ludwig-Maximilians University Munich (LMU)

Electrocardiogram (ECG) is the key variable to understanding cardiac electrophysiology. Physical exercise has beneficial effects but may also be harmful in the context of cardiovascular diseases. This manuscript provides a method of recording real-time ECG during exercise, which can serve to investigate its effects on cardiac electrophysiology in mice.

Biology

Three-Dimensional Imaging of Aortic Tissues in Atherosclerosis

Ting Sun^1,2,3, Mohammad R. Monjezi², Xu Xu², Changjun Yin^1,2,3,4, Andreas J.R. Habenicht^2,4, Sarajo K. Mohanta^2,4

¹Division of Vascular Surgery, The First Affiliated Hospital of Sun Yat-sen University, ²Institute for Cardiovascular Prevention (IPEK), Ludwig-Maximilians-University, ³Institute of Precision Medicine, The First Affiliated Hospital of Sun Yat-sen University, ⁴German Center for Cardiovascular Research (DZHK), Partner Site Munich Heart Alliance

Here, we present optimized tissue-clearing protocols to image the murine aorta in three dimensions (3D). We delineate state-of-the-art procedures for immunostaining, optical clearing, and imaging with the intent to define the anatomical proximity of the peripheral nervous system with atherosclerotic plaques and the adventitia in atherosclerosis.