Specimen Preparation, Imaging, and Analysis Protocols for Knife-edge Scanning MicroscopyYoonsuck Choe 1, David Mayerich 2, Jaerock Kwon 3, Daniel E. Miller 1, Chul Sung 1, Ji Ryang Chung 1, Todd Huffman 4, John Keyser 1, Louise C. Abbott 5
1Department of Computer Science and Engineering, Texas A&M University, 2Beckman Institute for Advanced Science and Technology, University of Illinois, 3Department of Electrical and Computer Engineering, Kettering University, 43Scan, 5Department of Veterinary Integrative Biosciences, Texas A&M University
The full process from brain specimen preparation to serial sectioning imaging using the Knife-Edge Scanning Microscope, to data visualization and analysis is described. This technique is currently used to acquire mouse brain data, but it is applicable to other organs, other species.
