Molloy College

1 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

Mesenchymal Stem Cell Regulation of Macrophage Phagocytosis; Quantitation and Imaging

Jodi F. Evans¹, Anthony E. Ricigliano¹, Anthony V. Morante¹, Emily Martinez¹, Darlisy Vargas¹, Jonah Thyagaraj¹

Presented here is a protocol to quantify and produce dynamic images of mesenchymal stem cell (MSC) mediated regulation of macrophage (MΦ) phagocytosis of non-opsonized yeast (zymosan) particles that are conjugated to a pH-sensitive fluorescent molecule.