Preparation of Washed Human Platelets for Quantitative Metabolic Flux StudiesSamantha Siska 1, Cara Sake 1, Keith B. Neeves 2,3, Nanette Boyle 1,4
1Depeartment of Chemical and Biological Engineering, Colorado School of Mines, 2Department of Bioengineering, University of Colorado Denver, Anschutz Medical Campus, 3Department of Pediatrics, Section of Hematology, Oncology, and Bone Marrow Transplant, University of Colorado Denver, Anschutz Medical Campus, 4Quantitative Biosciences and Engineering, Colorado School of Mines
Platelet metabolism is of interest, particularly as it relates to the role of platelet hyper- and hypoactivity in bleeding and thrombotic disorders. Isolating platelets from plasma is necessary for some metabolic assays; presented here is a method for isolating of intracellular metabolites from washed platelets.
