Centre for Molecular Biology at Heidelberg University (ZMBH)

1 ARTICLES PUBLISHED IN JoVE

Medicine

Isolation, Characterization, and Proteomic Analysis of Plasma-Derived Extracellular Vesicles for Cardiovascular Biomarker Discovery

Gabriel Jakob Trauner^*1, Dominika Bernath-Nagy^*1,2, Melek Sükran Kalinyaprak¹, Sabine Merker³, Marcin Luzarowski³, Florian Leuschner^1,4, Norbert Frey^1,4, Evangelos Giannitsis¹, Jona Benjamin Krohn^1,4

¹Department of Cardiology, Angiology and Pulmonology, University Hospital Heidelberg, ²Heart and Vascular Center, Semmelweis University, ³Core Facility for Mass Spectrometry and Proteomics, Centre for Molecular Biology at Heidelberg University (ZMBH), ⁴German Centre for Cardiovascular Research (DZHK), Partner site Heidelberg/Mannheim

This article describes a methodology for the isolation, characterization, and quantification of human plasma-derived extracellular vesicles (EV) and presents a workflow for label-free analysis of the EV proteome using liquid chromatography-tandem mass spectrometry (LC-MS/MS).