Cranial Neural Crest Cells Three-Dimensional In Vitro Differentiation Protocol for Multiplexed AssaySaverio Fortunato 1, Jean-Christophe Deschemin 1, Antoine Zalc 1
1Université Paris Cité, CNRS, Inserm, Institut Cochin
We present a three-dimensional (3D) in vitro differentiation protocol generating neurospheres of reproducible size to produce cranial neural crest cells from mouse embryonic stem cells. We show that this methodology reduces variability compared to previous protocols and how it can be used for multiplexed assay to study cranial neural crest cell development.