Droplet-based Cytotoxicity Assay to Assess Chimeric Antigen Receptor T cells at the Single-cell LevelZelvera Ismetova Usheva 1, David Leander Petersen 2, Torben Barington 1, Peter Mouritzen 2, Mike Bogetofte Barnkob 1
1Centre for Cellular Immunotherapy of Haematological Cancer Odense (CITCO), Department of Clinical Immunology, Odense University Hospital, 2Samplix ApS
Here, we describe a method for creating double-emulsion droplets encapsulating one T cell and a cancer target cell to examine cell killing at the single-cell level. This method allows for dual quantification of both cytotoxic molecules and target-cell apoptosis within a large population of T cells.
