Instituto Gulbenkian de Ciência

2 ARTICLES PUBLISHED IN JoVE

Developmental Biology

Three and Four-Dimensional Visualization and Analysis Approaches to Study Vertebrate Axial Elongation and Segmentation

André Dias¹, Gabriel G. Martins^1,2, Alexandre Lopes^1,3, Moisés Mallo¹

¹Instituto Gulbenkian de Ciência, ²Faculdade de Ciências da Universidade de Lisboa, ³NOVA School of Science and Technology

Here, we describe computational tools and methods that allow visualization and analysis of three and four-dimensional image data of mouse embryos in the context of axial elongation and segmentation, obtained by in toto optical projection tomography, and by live imaging and whole-mount immunofluorescence staining using multiphoton microscopy.

Cancer Research

Generation of Zebrafish Larval Xenografts and Tumor Behavior Analysis

Mayra Martinez-Lopez^1,2, Vanda Póvoa¹, Rita Fior¹

¹Champalimaud Centre for the Unknown, Champalimaud Foundation, ²Instituto Gulbenkian de Ciência

Here, we provide a step-by-step protocol, with tips to generate xenografts and guidelines for tumor behavior analysis, whole-mount immunofluorescence, and confocal imaging quantification.