In Vivo Confocal Fluorescence Imaging of Neural Activity Induced by Sensory Stimulation in Partially Restrained Larval ZebrafishJoseph B. Alzagatiti 1,2, Luis Salazar 3, Heidi Brown 3, Gabriel Rosas 3, Lama Adel Jaber 3, Jaime L. Minaya 3, Courtney Scaramella 4, Adam C. Roberts 3, David L. Glanzman 2,5,6
1Department of Molecular, Cellular, and Developmental Biology, University of California, Santa Barbara, 2Department of Integrative Biology and Physiology, University of California, Los Angeles, 3Department of Psychology, California State University at Fullerton, 4Department of Neuroscience, University of California, Riverside, 5Department of Neurobiology, David Geffen School of Medicine at UCLA, 6Integrative Center for Learning and Memory, Brain Research Institute, David Geffen School of Medicine at UCLA
Here, we present a detailed protocol to examine neural activity in brain regions of transgenic zebrafish that express GCaMP calcium indicators using confocal microscopy.
