Institut jacques Monod

1 ARTICLES PUBLISHED IN JoVE

Biochemistry

Using Microfluidics and Fluorescence Microscopy to Study the Assembly Dynamics of Single Actin Filaments and Bundles

Hugo Wioland¹, Foad Ghasemi¹, Jahnavi Chikireddy¹, Guillaume Romet-Lemonne¹, Antoine Jégou¹

¹Université Paris Cité, CNRS, Institut Jacques Monod

We present protocols for simple actin filament microfluidic assays, in combination with fluorescence microscopy, that allow one to accurately monitor individual actin filaments in real-time while sequentially exposing them to different protein solutions.