University Medical Center Hamburg-Eppendorf (UKE)

3 ARTICLES PUBLISHED IN JoVE

Medicine

Glutamate and Hypoxia as a Stress Model for the Isolated Perfused Vertebrate Retina

Kai Januschowski¹, Sebastian Müller¹, Carlo Krupp¹, Martin S. Spitzer¹, José Hurst¹, Maximilian Schultheiss¹, Karl-Ulrich Bartz-Schmidt¹, Peter Szurman¹, Sven Schnichels¹

¹Centre for Ophthalmology, University Eye Hospital Tübingen

With this study, we introduce a standardized stress model for the isolated superfused bovine retina for future preclinical therapeutic testing. The effect of either hypoxia (pure N₂) or glutamate stress (250 µM glutamate) on retinal function represented by a- and b-wave amplitudes was evaluated.

Medicine

A Porcine Corneal Endothelial Organ Culture Model Using Split Corneal Buttons

Daniel A. Wenzel¹, Berenike C. Kunzmann², Nils A. Steinhorst¹, Martin S. Spitzer¹, Maximilian Schultheiss¹

¹Department of Ophthalmology, University Medical Center Hamburg-Eppendorf (UKE), ²University Eye Hospital, Center For Ophthalmology, University Hospital Tübingen

Here, a step-by-step protocol for the preparation and cultivation of porcine split corneal buttons is presented. As this organo-typically cultivated organ culture model shows cell death rates within 15 days, comparable to human donor corneas, it represents the first model allowing long-term cultivation of non-human corneas without adding toxic dextran.

Immunology and Infection

Immunofluorescence Imaging of Neutrophil Extracellular Traps in Human and Mouse Tissues

Lavinia Schoenfeld¹, Birgit Appl¹, Laia Pagerols-Raluy¹, Annika Heuer³, Konrad Reinshagen¹, Michael Boettcher^1,2

¹Department of Pediatric Surgery, University Medical Center Hamburg-Eppendorf, University of Hamburg, ²Department of Pediatric Surgery, University Medical Center Mannheim, University of Heidelberg, ³Division of Spine Surgery, Department of Trauma and Orthopedic Surgery, University Medical Center Hamburg-Eppendorf (UKE)

Neutrophil extracellular traps (NETs) are associated with various diseases, and immunofluorescence is often used for their visualization. However, there are various staining protocols, and, in many cases, only one type of tissue is examined. Here, we establish a generally applicable protocol for staining NETs in mouse and human tissue.