MARINE BIOLOGICAL LABORATORY

5 ARTICLES PUBLISHED IN JoVE

Biology

Visualizing Cytoplasmic Flow During Single-cell Wound Healing in Stentor coeruleus

Mark Slabodnick^1,2, Bram Prevo^1,3, Peter Gross^1,4, Janet Sheung^1,5, Wallace Marshall^1,2

¹Physiology Course, Marine Biological Laboratory, ²Department of Biochemistry & Biophysics, University of California San Francisco, ³Department of Physics and Astronomy, Vrije Universiteit Amsterdam, ⁴Max Planck Institute of Molecular Cell Biology and Genetics, ⁵Department of Physics, University of Illinois Urbana-Champaign

The giant ciliate Stentor coeruleus is a classical system for studying regeneration and wound healing in single cells. By imaging Stentor cells simultaneously at low and high magnification it is possible to measure cytoplasmic flows before, during, and after wounding.

Biology

Fluorescence Imaging with One-nanometer Accuracy (FIONA)

Yong Wang^*1,2, En Cai^*1,2, Janet Sheung^1,2, Sang Hak Lee^1,2, Kai Wen Teng^2,3, Paul R. Selvin^1,2,3

¹Department of Physics, University of Illinois at Urbana-Champaign, ²Center for the Physics of Living Cells, University of Illinois at Urbana-Champaign, ³Center for Biophysics and Computational Biology, University of Illinois at Urbana-Champaign

Single fluorophores can be localized with nanometer precision using FIONA. Here a summary of the FIONA technique is reported, and how to carry out FIONA experiments is described.

Biology

Measurement of Survival Time in Brachionus Rotifers: Synchronization of Maternal Conditions

Gen Kaneko^1,4, Tatsuki Yoshinaga², Kristin E. Gribble³, David M. Welch³, Hideki Ushio¹

¹Department of Aquatic Bioscience, Graduate School of Agricultural and Life Sciences, The University of Tokyo, ²School of Marine Biosciences, Department of Marine Biosciences, Kitasato University, ³Josephine Bay Paul Center for Comparative Molecular Biology and Evolution, Marine Biological Laboratory, ⁴School of Arts and Sciences, University of Houston-Victoria

Rotifers are microscopic zooplankton used as models in ecotoxicological and aging studies. Here we provide a protocol for powerful and reproducible measurement of survival time in Brachionus rotifers. Synchronization of culture conditions over several generations is of particular importance because maternal condition affects life history of offspring.

Developmental Biology

Drosophila Embryo Preparation and Microinjection for Live Cell Microscopy Performed using an Automated High Content Analyzer

Ulises Diaz^1,2, Wallace Marshall², Blake Riggs¹

¹Department of Biology, San Francisco State University, ²Department of Biochemistry & Biophysics, UCSF Mission Bay

Presented here is a protocol to microinject and simultaneously image multiple Drosophila embryos during embryonic development using a plate-based, high content imager.

Biology

Studying Habituation in Stentor coeruleus

Deepa Rajan¹, Peter Chudinov¹, Wallace Marshall¹

¹Department of Biochemistry and Biophysics, University of California San Francisco

We introduce a method for quantifying Stentor habituation using a microcontroller board-linked apparatus that can deliver mechanical pulses at a specified force and frequency. We also include methods for assembling the apparatus and setting up the experiment in a way that minimizes external perturbations.