Quantitative and Automated High-throughput Genome-wide RNAi Screens in C. elegansBarbara Squiban 1, Jérôme Belougne 1, Jonathan Ewbank 1, Olivier Zugasti 1
1Centre d’Immunologie de Marseille-Luminy, Université de la Méditerranée
We describe a protocol using C. elegans and RNAi feeding libraries that allows automated measurement of multiple parameters such as fluorescence, size and opacity of individual worms in a population. We give one example of a screen to identify genes involved in anti-fungal innate immunity in C. elegans.
