National Institute of Infectious Diseases

2 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

Particle Agglutination Method for Poliovirus Identification

Minetaro Arita¹, Souji Masujima², Takaji Wakita¹, Hiroyuki Shimizu¹

¹Department of Virology II, National Institute of Infectious Diseases, ²New Product Design Department, Fujirebio Inc.

A recently developed novel particle agglutination (PA) assay utilizing virus receptor molecule allowed a rapid and easy identification of poliovirus (PV). In this article, we will show the procedure for the PA assay for PV identification.

Immunology and Infection

Identification of Mouse and Human Antibody Repertoires by Next-Generation Sequencing

Lin Sun¹, Naoko Kono², Hiroyuki Toh³, Hanbing Xue¹, Kaori Sano^4,5, Tadaki Suzuki⁴, Akira Ainai⁴, Yasuko Orba⁶, Junya Yamagishi^7,8, Hideki Hasegawa^4,5, Yoshimasa Takahashi⁹, Shigeyuki Itamura², Kazuo Ohnishi^9,10

¹Graduate School of Life and Environmental Sciences, University of Tsukuba, ²Center for Influenza Virus Research, National Institute of Infectious Diseases, ³School of Science and Technology, Kwansei Gakuin University, ⁴Department of Pathology, National Institute of Infectious Diseases, ⁵Division of Infectious Diseases Pathology, Department of Global Infectious Diseases, Tohoku University Graduate School of Medicine, ⁶Division of Molecular Pathobiology, Research Center for Zoonosis Control, Hokkaido University, ⁷Division of Collaboration and Education, Research Center for Zoonosis Control, Hokkaido University, ⁸Global Station for Zoonosis Control, GI-CoRE, Hokkaido University, ⁹Department of Immunology, National Institute of Infectious Diseases, ¹⁰Faculty of Life and Environmental Sciences, University of Tsukuba

Here, we describe protocols for the analysis and visualization of the structure and constitution of whole antibody repertoires. This involves the acquisition of vast sequences of antibody RNA using next-generation sequencing.