University of Lübeck

6 ARTICLES PUBLISHED IN JoVE

Medicine

A Human Fallopian Tube Model for Investigation of C. trachomatis Infections

Stefan Jerchel¹, Gudrun Knebel², Peter König², Michael K. Bohlmann³, Jan Rupp^1,4

¹Institute of Medical Microbiology and Hygiene, University of Lübeck, ²Institute of Anatomie, University of Lübeck, ³Department of Obstetrics and Gynecology, University Hospital of Schleswig-Holstein, University of Lübeck, ⁴Medical Clinic III, University Hospital of Schleswig-Holstein, University of Lübeck

We describe an ex vivo infection model for visualisation of direct interactions from bacterial pathogens with human fallopian tube cells. The whole organ tissue model was established to investigate C. trachomatis induced pathology to the female fallopian tube under "life-like" conditions.

Bioengineering

A Full Skin Defect Model to Evaluate Vascularization of Biomaterials In Vivo

Thilo L. Schenck¹, Myra N. Chávez¹, Alexandru P. Condurache², Ursula Hopfner¹, Farid Rezaeian³, Hans-Günther Machens¹, José T. Egaña^1,4

¹Department of Plastic Surgery and Hand Surgery, University Hospital rechts der Isar, Technische Universität München, ²Institute for Signal Processing, University of Lübeck, ³Department of Plastic Surgery and Hand Surgery, University Hospital Zürich, ⁴FONDAP Center for Genome Regulation, Facultad de Ciencias, Universidad de Chile

Vascularization is key to approaches in successful tissue engineering. Therefore, reliable technologies are required to evaluate the development of vascular networks in tissue-constructs. Here we present a simple and cost-effective method to visualize and quantify vascularization in vivo.

Engineering

Robotic Sensing and Stimuli Provision for Guided Plant Growth

Mostafa Wahby¹, Mary Katherine Heinrich^1,5, Daniel Nicolas Hofstadler², Julian Petzold¹, Igor Kuksin³, Payam Zahadat^2,4, Thomas Schmickl², Phil Ayres⁵, Heiko Hamann¹

¹Institute of Computer Engineering, University of Lübeck, ²Institute of Biology, Artificial Life Lab, University of Graz, ³Cybertronica UG, ⁴Department of Computer Science, IT University of Copenhagen, ⁵Centre for IT and Architecture, Royal Danish Academy

Distributed robot nodes provide sequences of blue light stimuli to steer the growth trajectories of climbing plants. By activating natural phototropism, the robots guide the plants through binary left-right decisions, growing them into predefined patterns that by contrast are not possible when the robots are dormant.

Medicine

Development of a Noninvasive, Laser-Assisted Experimental Model of Corneal Endothelial Cell Loss

Annekatrin Holzhey^*1, Svenja Sonntag^*2, Johannes Rendenbach¹, Justus S. Ernesti¹, Vinodh Kakkassery², Salvatore Grisanti², Fred Reinholz³, Sebastian Freidank³, Alfred Vogel³, Mahdy Ranjbar^1,2

¹Laboratory for Angiogenesis and Ocular Cell Transplantation, University of Lübeck, ²Department of Ophthalmology, University of Lübeck, ³Institute of Biomedical Optics, University of Lübeck

Here, we present a protocol to detach corneal endothelial cells (CEC) from Descemet’s membrane (DM) using a neodymium:YAG (Nd:YAG) laser as an ex vivo disease model for bullous keratopathy (BK).

Medicine

Organotypic Slice Cultures as Preclinical Models of Tumor Microenvironment in Primary Pancreatic Cancer and Metastasis

Rüdiger Braun¹, Olha Lapshyna¹, Susanne Eckelmann^2,3, Kim Honselmann¹, Louisa Bolm¹, Meike ten Winkel¹, Steffen Deichmann¹, Oliver Schilling⁴, Charli Kruse^2,3, Tobias Keck¹, Ulrich Wellner¹, Peter Bronsert^4,5,6, Matthias Brandenburger³

¹Department of Surgery, University Medical Center Schleswig-Holstein, ²Institute of Medical and Marine Biotechnology, University of Lübeck, ³Fraunhofer Research and Development Center for Marine and Cellular Biotechnology, ⁴Institute for Surgical Pathology, Medical Center - University of Freiburg, Faculty of Medicine, University of Freiburg, ⁵Tumorbank Comprehensive Cancer Center Freiburg, Medical Center - University of Freiburg, ⁶Core Facility Histopathology and Digital Pathology Freiburg, Medical Center - University of Freiburg

This protocol describes the preparation of organotypic slice cultures (OTSCs). This technique facilitates the ex vivo cultivation of intact multicellular tissue. OTSCs can be used immediately to test for their respective response to drugs in a multicellular environment.

Neuroscience

Topographical EEG Recordings of Visual Evoked Potentials in Mice using Multichannel Thin-film Electrodes

Rüdiger Land¹, Sarah Christine Sentis^1,2, Andrej Kral¹

¹Institute for Audioneurotechnology, Department of Experimental Otology, Hannover Medical School, ²Institute for Endocrinology and Diabetes, Center of Brain, Behavior and Metabolism, University of Lübeck

The present protocol describes a simple procedure to acquire and analyze the topography of epicranial visual evoked potentials with 32-multichannel thin-film electrodes in the mouse.