National Cancer Institute - Frederick
2 ARTICLES PUBLISHED IN JoVE
Biology
Isolation of Immune Cells from Primary Tumors
Stephanie K. Watkins 1, Ziqiang Zhu 1, Keith E. Watkins, Arthur A. Hurwitz 1
1Tumor Immunity and Tolerance Section, Laboratory of Molecular Immunoregulation, Cancer and Inflammation Program, National Cancer Institute - Frederick
In this report, we describe a protocol for isolating highly purified populations of leukocytes that infiltrate tumors. This protocol is adapted from the Miltenyi Biotech protocol to enhance yield and purity for isolating cells from complex tumor tissue.
Biology
Luciferase Complementation Imaging Assay in Nicotiana benthamiana Leaves for Transiently Determining Protein-protein Interaction Dynamics
Kaiwen Sun *1, Yuyu Zheng *1, Ziqiang Zhu 1
1Jiangsu Key Laboratory for Biodiversity and Biotechnology, College of Life Sciences, Nanjing Normal University
This manuscript describes an easy and rapid experimental procedure for determining protein-protein interactions based on the measurement of luciferase activity.
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