The Walter & Eliza Hall Institute of Medical Research
2 ARTICLES PUBLISHED IN JoVE
Immunology and Infection
Using Bioluminescent Imaging to Investigate Synergism Between Streptococcus pneumoniae and Influenza A Virus in Infant Mice
Kirsty R. Short 1, Dimitri A. Diavatopoulos 2, Patrick C. Reading 1, Lorena E. Brown 1, Kelly L. Rogers 3, Richard A. Strugnell 1, Odilia L.C. Wijburg 1
1Department of Microbiology and Immunology, University of Melbourne, 2Laboratory of Pediatric Infectious Diseases, Radboud University Nijmegen Medical Centre, 3The Centre for Dynamic Imaging, The Walter and Eliza Hall Institute for Medical Research
A concurrent infection with influenza A virus is one of the factors implicated in the induction of invasive pneumococcal disease during asymptomatic Streptococcus pneumoniae carriage. Here we describe a mixed infection method using infant mice to investigate the synergism between these two respiratory pathogens.
Bioengineering
A Step Beyond BRET: Fluorescence by Unbound Excitation from Luminescence (FUEL)
Joseph Dragavon 1, Carolyn Sinow 2, Alexandra D. Holland 1, Abdessalem Rekiki 1, Ioanna Theodorou 3, Chelsea Samson 4, Samantha Blazquez 1, Kelly L. Rogers 5, Régis Tournebize 1,6,7, Spencer L. Shorte 1
1Plate-Forme d'Imagerie Dynamique, Imagopole, Institut Pasteur, 2Department of Radiation Oncology, Stanford School of Medicine, 3Service Hospitalier Frédéric Joliot, Institut d'Imagerie Biomédicale, 4Vanderbilt School of Medicine, 5The Walter & Eliza Hall Institute of Medical Research, 6Unité INSERM U786, Institut Pasteur, 7Unité de Pathogénie Microbienne Moléculaire, Institut Pasteur
Expanding the foundation and applicability of Fluorescence by Unbound Excitation from Luminescence (FUEL) by surveying the relevant principles and demonstrating its compatibility with a multitude of fluorophores and antibody-targeted conditions.
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