University of Saskatchewan

2 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

Tracking Neutrophil Intraluminal Crawling, Transendothelial Migration and Chemotaxis in Tissue by Intravital Video Microscopy

Najia Xu¹, Xi Lei¹, Lixin Liu¹

¹Department of Pharmacology, University of Saskatchewan

We describe a protocol of brightfield intravital microscopy for measuring dynamic neutrophil-endothelial cell interactions during neutrophil recruitment in response to the source of a neutrophil chemoattractant in vivo. Neutrophil intraluminal crawling, transendothelial migration and chemotaxis in mouse cremaster muscle tissue are visualized with time-lapsed video photography and tracked with ImageJ.

Immunology and Infection

Multiplex Detection of Bacteria in Complex Clinical and Environmental Samples using Oligonucleotide-coupled Fluorescent Microspheres

Tim J. Dumonceaux¹, Jennifer R. Town¹, Janet E. Hill², Bonnie L. Chaban², Sean M. Hemmingsen³

¹Saskatoon Research Centre, Agriculture and Agri-Food Canada, ²Department of Veterinary Microbiology, University of Saskatchewan , ³Plant Biotechnology Institute, National Research Council of Canada

We describe a multiplex method for the detection of microorganisms within a sample using oligonucleotide-coupled fluorescent beads. Amplicon from all organisms within a sample is hybridized to a panel of probe-coupled beads. A Luminex or Bio-Plex instrument is used to query each bead for bead type and hybridization signal.