Morehouse School of Medicine
2 ARTICLES PUBLISHED IN JoVE
JoVE Journal
Peptide-based Identification of Functional Motifs and their Binding Partners
Martin N. Shelton 1,2, Ming Bo Huang 1, Syed Ali 1,3, Kateena Johnson 1, William Roth 1, Michael Powell 1, Vincent Bond 1
1Department of Microbiology, Biochemistry, & Immunology, Morehouse School of Medicine, 2Institute for Systems Biology, 3Advanced Medical & Dental Institute, Universiti Sains Malaysia
Techniques to dissect the mechanisms underlying the secretion of HIV-1 Nef in exosomes are described. Specific short peptides derived from Nef and protein transfection were exploited to determine the structure, function, and binding partners of Nef’s Secretion Modification Region. These procedures have general relevance in many mechanistic studies.
JoVE Journal
Isolation of Exosomes from the Plasma of HIV-1 Positive Individuals
Kateena Addae Konadu 1, Ming Bo Huang 1, William Roth 1, Wendy Armstrong 2, Michael Powell 1, Francois Villinger 3,4, Vincent Bond 1
1Department of Microbiology, Biochemistry, Immunology, Morehouse School of Medicine, 2Department of Medicine, Emory University School of Medicine, 3Department of Pathology and Laboratory Medicine, Emory University School of Medicine, 4Yerkes National Primate Research Center
Techniques describing a gradient procedure to separate exosomes from human immunodeficiency virus (HIV) particles are described. This procedure was used to isolate exosomes away from HIV particles in human plasma from HIV-infected individuals. The isolated exosomes were analyzed for cytokine/chemokine content.
