Morehouse School of Medicine

2 ARTICLES PUBLISHED IN JoVE

JoVE Journal

Peptide-based Identification of Functional Motifs and their Binding Partners

Martin N. Shelton^1,2, Ming Bo Huang¹, Syed Ali^1,3, Kateena Johnson¹, William Roth¹, Michael Powell¹, Vincent Bond¹

¹Department of Microbiology, Biochemistry, & Immunology, Morehouse School of Medicine, ²Institute for Systems Biology, ³Advanced Medical & Dental Institute, Universiti Sains Malaysia

Techniques to dissect the mechanisms underlying the secretion of HIV-1 Nef in exosomes are described. Specific short peptides derived from Nef and protein transfection were exploited to determine the structure, function, and binding partners of Nef’s Secretion Modification Region. These procedures have general relevance in many mechanistic studies.

JoVE Journal

Isolation of Exosomes from the Plasma of HIV-1 Positive Individuals

Kateena Addae Konadu¹, Ming Bo Huang¹, William Roth¹, Wendy Armstrong², Michael Powell¹, Francois Villinger^3,4, Vincent Bond¹

¹Department of Microbiology, Biochemistry, Immunology, Morehouse School of Medicine, ²Department of Medicine, Emory University School of Medicine, ³Department of Pathology and Laboratory Medicine, Emory University School of Medicine, ⁴Yerkes National Primate Research Center

Techniques describing a gradient procedure to separate exosomes from human immunodeficiency virus (HIV) particles are described. This procedure was used to isolate exosomes away from HIV particles in human plasma from HIV-infected individuals. The isolated exosomes were analyzed for cytokine/chemokine content.