Juniata College

3 ARTICLES PUBLISHED IN JoVE

Environment

A Simple Approach to Manipulate Dissolved Oxygen for Animal Behavior Observations

Christopher J. Grant¹, Christopher J. McLimans¹

This article describes a simple and reproducible protocol to manipulate dissolved oxygen conditions in a laboratory setting for animal behavior studies. This protocol may be used in both teaching and research laboratory settings to evaluate organismal response of macroinvertebrates, fishes, or amphibians to changes in dissolved oxygen concentration.

Neuroscience

Ocular Kinematics Measured by In Vitro Stimulation of the Cranial Nerves in the Turtle

Maria Cano Garcia¹, Steven C. Nesbit¹, Chi C. Le², James R. Dearworth Jr.¹

¹Department of Biology and Neuroscience Program, Lafayette College, ²Department of Information Technology, Computer Science, and Digital Media, Juniata College

This protocol describes how to use an in vitro isolated turtle head preparation to measure the kinematics of their eye movements. After removal of the brain from the cranium, cranial nerves can be stimulated with currents to quantify rotations of the eye and changes in pupil sizes.

Environment

Evaluating the Impact of Hydraulic Fracturing on Streams using Microbial Molecular Signatures

Jeremy R. Chen See^1,2, Olivia Wright¹, Lavinia V. Unverdorben^1,2, Nathan Heibeck¹, Stephen M. Techtmann³, Terry C. Hazen^4,5, Regina Lamendella^1,2

¹Department of Biology, Juniata College, ²Wright Labs, LLC, ³Department of Biological Sciences, Michigan Technological University, ⁴Biosciences Division, Oak Ridge National Laboratory, ⁵Department of Civil and Environmental Engineering, University of Tennessee

Here, we present a protocol to investigate the impacts of hydraulic fracturing on nearby streams by analyzing their water and sediment microbial communities.