Institute for Systems Biology

4 ARTICLES PUBLISHED IN JoVE

Biology

Mechanical Stimulation of Stem Cells Using Cyclic Uniaxial Strain

¹Department of Bioengineering, University of California, Berkeley

It is widely understood that mechanical forces in the body can influence cell differentiation and proliferation. Here we present a video protocol demonstrating the use of a custom-built bioreactor for delivering uniaxial cyclic tensile strain to stem cells cultured on flexible micropatterned substrates.

Biology

Quantitative Phosphoproteomics in Fatty Acid Stimulated Saccharomyces cerevisiae

Ramsey A. Saleem¹, John D. Aitchison¹

¹Institute for Systems Biology

Description of a quantitative phosphorylation procedure using cryolysis, urea solubilziation, HILIC fractionation and IMAC enrichment of phosphorylated peptides.

JoVE Journal

Peptide-based Identification of Functional Motifs and their Binding Partners

Martin N. Shelton^1,2, Ming Bo Huang¹, Syed Ali^1,3, Kateena Johnson¹, William Roth¹, Michael Powell¹, Vincent Bond¹

¹Department of Microbiology, Biochemistry, & Immunology, Morehouse School of Medicine, ²Institute for Systems Biology, ³Advanced Medical & Dental Institute, Universiti Sains Malaysia

Techniques to dissect the mechanisms underlying the secretion of HIV-1 Nef in exosomes are described. Specific short peptides derived from Nef and protein transfection were exploited to determine the structure, function, and binding partners of Nef’s Secretion Modification Region. These procedures have general relevance in many mechanistic studies.

Biology

ODELAY: A Large-scale Method for Multi-parameter Quantification of Yeast Growth

Thurston Herricks¹, Fred D. Mast^1,2, Song Li¹, John D. Aitchison^1,2

¹Institute for Systems Biology, ²Center for Infectious Disease Research

We present a method for quantifying growth phenotypes of individual yeast cells as they grow into colonies on solid media using time-lapse microscopy termed, One-cell Doubling Evaluation of Living Arrays of Yeast (ODELAY). Population heterogeneity of genetically identical cells growing into colonies can be directly observed and quantified.