Fluorescence Lifetime Imaging of Molecular Rotors in Living CellsKlaus Suhling 1, James A. Levitt 1, Pei- Hua Chung 1, Marina. K. Kuimova 2, Gokhan Yahioglu 3
1Department of Physics, King's College London, 2Department of Chemistry, Imperial College London , 3PhotoBiotics Ltd
Fluorescence Lifetime Imaging (FLIM) has emerged as a key technique to image the environment and interaction of specific proteins and dyes in living cells. FLIM of fluorescent molecular rotors allows mapping of viscosity in living cells.
