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xi'an jiaotong university

8 ARTICLES PUBLISHED IN JoVE

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Cancer Research

Sample Extraction and Simultaneous Chromatographic Quantitation of Doxorubicin and Mitomycin C Following Drug Combination Delivery in Nanoparticles to Tumor-bearing Mice
Rui Xue Zhang 1, Tian Zhang 1, King Chen 1, Ji Cheng 1, Paris Lai 1, Andrew M. Rauth 2, K. Sandy Pang 1, Xiao Yu Wu 1
1Department of Pharmaceutical Sciences, University of Toronto, 2Departments of Medical Biophysics and Radiation Oncology, University of Toronto, Ontario Cancer Institute, University Health Network

This protocol describes an efficient and convenient analytical process of sample extraction and simultaneous determination of multiple drugs, doxorubicin (DOX), mitomycin C (MMC) and a cardio-toxic DOX metabolite, doxorubicinol (DOXol), in the biological samples from a preclinical breast tumor model treated with nanoparticle formulations of synergistic drug combination.

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Medicine

Murine Appendectomy Model of Chronic Colitis Associated Colorectal Cancer by Precise Localization of Caecal Patch
Yaguang Li 1, Junhong Liu 2,3, Gaixia Liu 1, Zhenhong Pan 2,3, Mingxia Zhang 1, Yao Ma 4, Qingxia Wei 1,5, Hongping Xia 1,6, Rui Xue Zhang 1,2, Junjun She 1
1Department of General Surgery, The First Affiliated Hospital, Xi'an Jiaotong University, 2Institute of Medical Research, Northwestern Polytechnical University, 3School of Life Sciences, Northwestern Polytechnical University, 4Department of Medicine, Xi'an Jiaotong University, 5Princess Margaret Cancer Center, University of Health Network, 6Department of Pathology, Nanjing Medical University

The presented protocol describes a facile surgical removal of the appendix (caecal patch) in a mouse followed by the induction of inflammatory bowel disease-associated colorectal cancer. This murine appendectomy model enables investigation of the biological role of the appendix in the pathogenesis of human gastrointestinal disease.

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Neuroscience

Use of Primary Cultured Hippocampal Neurons to Study the Assembly of Axon Initial Segments
Rui Yang 1,2,3, Vann Bennett 2,3
1School of Basic Medical Sciences, Xi'an Jiaotong University, 2Department of Cell Biology, Duke University, 3Department of Biochemistry, Duke University

Here, we described a protocol to quantitatively study the assembly and structure of the axon initial segments (AIS) of hippocampal neurons that lack pre-assembled AIS due to the absence of a giant ankyrin-G.

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Neuroscience

Craniotomy Procedure for Visualizing Neuronal Activities in Hippocampus of Behaving Mice
YangDong Wang 1,2,3,4, DanYang Zhu 5, BaoYue Liu 1,2,3,6, Kiryl D. Piatkevich 1,2,3
1School of Life Sciences, Westlake University, 2Westlake Laboratory of Life Sciences and Biomedicine, 3Institute of Basic Medical Sciences, Westlake Institute for Advanced Study, 4School of Basic Medical Sciences, Xi'an JiaoTong University, 5Office of Public Affairs, Westlake University, 6School of Pharmaceutical Sciences, Jilin University

This article demonstrates the preparation of a custom-made imaging window supplemented with infusion cannula and its implantation onto the CA1 region of the hippocampus in mice.

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Medicine

Robot-assisted Total Mesorectal Excision and Lateral Pelvic Lymph Node Dissection for Locally Advanced Middle-low Rectal Cancer
Chenhao Hu *1,2,3, Zhe Zhang *1,2,3, Lei Zhang 1,2,3, Ruihan Liu 2,3, Jun Yan 1,2,3, Qi Sun 1,2,3, Guanghui Wang 1, Junjun She 1,2,3
1Department of General Surgery, The First Affiliated Hospital of Xian Jiaotong University, 2Center for Gut Microbiome Research, Med-X Institute, The First Affiliated Hospital of Xian Jiao tong University, 3Department of High Talent, The First Affiliated Hospital of Xian Jiaotong University

The robotic technique described herein aims to detail a stepwise approach to robot-assisted total mesorectal excision and lateral pelvic lymph node dissection for locally advanced (T3/T4) rectal cancer located below the peritoneal reflection.

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Medicine

Microsurgical Skills of Establishing Permanent Jugular Vein Cannulation in Rats for Serial Blood Sampling of Orally Administered Drug
Weijia Lu *1, Ruimin Miao *1, Sijun Hu 1, Junhong Liu 1, Fanqi Jin 1, Rui Xue Zhang 1
1Xi'an Key Laboratory of Stem Cell and Regenerative Medicine, Institute of Medical Research, Northwestern Polytechnical University

Detailed microsurgical techniques are demonstrated to establish a longer-term jugular vein cannulation rat model for sequential blood collection in the same animal. Physiological and hematological parameters have been monitored during the rat's recovery phase. This model has been applied to study pharmacokinetics of orally administered polyphenol without inducing animal stress.

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Medicine

Orthopedic Robot-Assisted Femoral Neck System in the Treatment of Femoral Neck Fracture
Yuxuan Cong *1, Pengfei Wen *2, Yongchao Duan 3, Hai Huang 1, Yan Zhuang 1, Pengfei Wang 1
1Department of Orthopedic Trauma, HongHui Hospital, Xi'an Jiaotong University, 2Department of Joint Surgery, HongHui Hospital, Xi'an Jiaotong University, 3Department of Intraoperative Imaging, HongHui Hospital, Xi'an Jiaotong University

This article introduces a method of robot-assisted orthopedic surgery for screw placement during the treatment of femoral neck fracture using the femoral neck system, which allows for more accurate screw placement, improved surgical efficiency, and fewer complications.

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Engineering

Microembossing: A Convenient Process for Fabricating Microchannels on Nanocellulose Paper-Based Microfluidics
Wenwen Yuan 1,2,3, Hang Yuan 1, Sixuan Duan 1,2, Ruiqi Yong 1, Jia Zhu 1,2,4, Eng Gee Lim 1,2, Ivona Mitrovic 2, Pengfei Song 1,2
1School of Advanced Technology, Xi’an Jiaotong - Liverpool University, 2Department of Electrical Engineering and Electronics, University of Liverpool, 3State Key Laboratory for Manufacturing Systems Engineering, Xi'an Jiaotong University, 4School of Intelligent Manufacturing and Smart Transportation, Suzhou City University

This protocol describes a straightforward process that utilizes convenient plastic micro-molds for simple microembossing operations to fabricate microchannels on nanofibrillated cellulose paper, achieving a minimum width of 200 µm.

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