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Nihon Pharmaceutical University

1 ARTICLES PUBLISHED IN JoVE

Biochemistry

The Determination of Protease Specificity in Mouse Tissue Extracts by MALDI-TOF Mass Spectrometry: Manipulating PH to Cause Specificity Changes

Hiroyuki Yamamoto¹, Yoshikazu Sawaguchi², Michio Kimura¹

¹Department of Microbiology and Molecular Cell Biology, Nihon Pharmaceutical University, ²Department of Clinical Pharmaceutics, Nihon Pharmaceutical University

Here, we present a protocol to determine protease specificity in crude mouse tissue extracts using MALDI-TOF spectrometry.

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